Anlysis in Biochmeistry Lab Report Example | Topics and Well Written Essays - 1000 words

Anlysis in Biochmeistry - Lab Report Example The risk can be minimsed by using reliable controls for background fluorescence check as is used in the current study. The average percentage inhibition of the cytochrome P450 3A4 by ketaconazole was determined using a fluorescence based enzyme assay. The readings were obtained as per procedure and is recorded and calculated in Tables 1, 2, 3 and 4. Ketaconazole samples in varying concentrations showed lower fluorescence than the sample without the test compound indicating the inhibitory activity of the drug on the enzyme cytochrome P450. Saple with 10 M concentration showed the least fluorescence and therefore the highest % inhibition of 95.274.7 and even a lower concentration of 0.1 M also shows a percentage inhibition of about 75.616.12. The fluorescence reading shows the inhibition of the enzyme to be directly proportional to the concentration of the test compound ketaconazole. The present study a fluorescence based assay to illustrate and evaluate the interaction of ketaconazole with the liver cytochrome P450 A4 using microsomes prepared from insect cells that express both human P450 isozyme and rabbit NADPH-P450 reductase and a substrate th